Anti-MMP-9 (Ab-3) Mouse mAb (56-2A4)

Analysis Note

Negative ControlMMP-2 protein (Cat. Nos. PF023 or PF037)

Positive ControlMMP-9 protein (Cat. Nos. PF024 or PF038) or breast carcinoma tissue

Application

Frozen Sections (see application references)Immunoblotting (1 g/ml)Paraffin Sections (see application references)

General description

Purified mouse monoclonal antibody (see application references). Recognizes both the ~92 kDa latent and the ~83 kDa active forms of MMP-9.

Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 in breast carcinoma tissue.

This Anti-MMP-9 (Ab-3) Mouse mAb (56-2A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-9 (Ab-3).

Immunogen

Human

a synthetic peptide corresponding to amino acids 626-644 of human MMP-9

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol.2, 861.Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.Okada, Y., et al., 1992. J. Biol. Chem.267, 21712.Woessner, J.F. 1991. FASEB J.5, 2145.Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.

This antibody does not react with MMP-1, MMP-2, MMP-3 or MMP-13. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)